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While doing a PCR, ‘denaturation’ step is missed. What will be
Biology Exemplar
Biology Exemplar

Class 12

NCERT Exemplar

Publisher:
NCERT
Subject:
Biology
1

Chapter 1: Reproduction in Organisms

48 questions

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2

Chapter 2: Sexual Reproduction in Flowering Plants

52 questions

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3

Chapter 3: Human Reproduction

48 questions

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4

Chapter 4: Reproductive Health

37 questions

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5

Chapter 5: Principles of Inheritance and Variation

52 questions

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6

Chapter 6: Molecular Basis of Inheritance

71 questions

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7

Chapter 7: Evolution

52 questions

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8

Chapter 8: Human Health and Disease

70 questions

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9

Chapter 9: Strategies for Enhancement in Food Production

77 questions

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10

Chapter 10: Microbes in Human Welfare

59 questions

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11

Chapter 11: Biotechnology: Principles and Processes

48 questions

Q1
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Q2
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Q3
What modification is done on the Ti-plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
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12

Chapter 12: Biotechnology and its Applications

52 questions

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13

Chapter 13: Organisms and Populations

70 questions

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14

Chapter 14: Ecosystem

68 questions

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15

Chapter 15: Biodiversity and Conservation

61 questions

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16

Chapter 16: Environmental Issues

52 questions

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While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

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While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
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If denaturation of double-stranded DNA does not take place then primers will not be able to anneal (joining) to the template. Hence, no extension will take place and after are there will be no amplification.
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Question Text
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
TopicBiotechnology: Principles and Processes
SubjectBiology
ClassClass 12
Answer TypeText solution:1
Upvotes138