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tetracycline containing medium. The transformants growing on ampicilli

tetracycline containing medium. The transformants growing on ampicillin containing medium are then transferred on a medium containing tetracycline. The recombinants will grow in ampicillin containing medium but not on that containing tetracycline. But, non- recombinants will grow on the medium containing both the antibiotics. In this case, one antibiotic resistance gene helps in selecting the transformants, whereas the other antibiotic resistance Uttarakhand Mudran Varsh 2018-19

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Biotechnology - Principles and Processes 41 Although a genomic library represent the entire genome of an organism, it may not be useful in case of eukaryotic organisms. Genomic DNA of eukaryotes contains non-coding DNA like introns, control regions and repetitive sequences unrecognized by prokaryotic systems such as in E. coli. Therefore another library called cDNA library is preferred especially if the aim of the cloning experiment is to express eukaryotic proteins in an E. coli host. This library has two major advantages over a genomic library. Firstly, it represents only those genes that are being expressed by a particular cell or under specific conditions. Eukaryotes, which include multicellular organisms such as animals and plants have differentiated cells which means that different cells e.g. liver and brain cells express some common proteins and also other proteins which are not common. Secondly, since the source material in constructing such libraries is mRNA, these molecules lack introns and hence would represent only the coding sequence of the genome. However mRNA molecules are highly unstable as they are easily degraded by RNAses and hence these molecules are faithfully copied into the more stable DNA (now called cDNA) before cloning. The construction of a cDNA library begins with the isolation of mRNA from a given cell type or tissue which are copied into cDNA using a special enzyme called reverse transcriptase. The procedure results in double-stranded cDNA which can be incorporated into vectors such as pBR322.
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tetracycline containing medium. The transformants growing on ampicillin containing medium are then transferred on a medium containing tetracycline. The recombinants will grow in ampicillin containing medium but not on that containing tetracycline. But, non- recombinants will grow on the medium containing both the antibiotics. In this case, one antibiotic resistance gene helps in selecting the transformants, whereas the other antibiotic resistance Uttarakhand Mudran Varsh 2018-19
Updated OnNov 15, 2022
TopicBiotechnology
SubjectBiology
ClassClass 12
Answer Type Video solution: 1
Upvotes60
Avg. Video Duration13 min