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A plasmid DNA and a linear DNA (both are of the same size) hav
Biology Exemplar
Biology Exemplar

Class 12

NCERT Exemplar

Publisher:
NCERT
Subject:
Biology
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11

Chapter 11: Biotechnology: Principles and Processes

48 questions

Q1
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
Q2
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA b and, while linear DNA shows two fragments. Explain.
Q3
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
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Chapter 12: Biotechnology and its Applications

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Chapter 13: Organisms and Populations

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Chapter 14: Ecosystem

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Chapter 15: Biodiversity and Conservation

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Chapter 16: Environmental Issues

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A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA b and, while linear DNA shows two fragments. Explain.

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Updated on: Dec 21, 2022

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A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA b and, while linear DNA shows two fragments. Explain.
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When a plasmid DNA and a linear DNA having one site for a restriction endonuclease are cut and separated, plasmid shows one DNA band, while linear DNA shows two band because of difference in their basic structure.
Plasmid is a circular DNA molecule and when cut with these enzyme, it becomes linear but does not get fragmented due to presence of only one restriction site, whereas a linear DNA molecule gets cut into two fragment.
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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
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Question Text
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA b and, while linear DNA shows two fragments. Explain.
Updated OnDec 21, 2022
TopicBiotechnology: Principles and Processes
SubjectBiology
ClassClass 12
Answer TypeText solution:1 Video solution: 1
Upvotes144
Avg. Video Duration2 min