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Question 11
Hard
Views

Views: 5,713

Three identical potato cylinders were used to investigate water movement in plant cells.
Cranberry juice is a red fruit juice that contains natural sugars.
Three test-tubes were set up as shown in figure and left for one hour.

After one hour the potato cylinders were removed from test-tubes 1 to 3.
The mass of each potato cylinder is recorded in the table.

(i) Describe the results for test-tubes 1 and 2.
test-tube 1 .........................................................................................................................
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test-tube 2 .........................................................................................................................
...........................................................................................................................................
(ii) Calculate the decrease in the mass of the potato cylinder in test-tube 3.
........................................................g
(iii) Explain why the potato cylinder lost mass in test-tube 3.
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Question 13
Hard
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Views: 5,666

You are going to measure the distance moved by different concentrations of citric acid through agar.

You are provided with a Petri dish labelled agar plate.

The agar in the Petri dish contains Universal Indicator which will change colour in the presence of acid.

You should use the safety equipment provided while you are carrying out the practical work.
Step 1 Label three test-tubes A, B and C and place them in a test-tube rack.
Step 2 Make three solutions, each containing a different concentration of citric acid, in the labelled test-tubes.
Use the volumes of 5% citric acid and distilled water shown in the table to make the solutions.

Step 3 Turn the Petri dish over so the base side is up. Use a marker pen to draw three lines to divide the base into approximately equal sections. Label the sections A, B and C as shown in Fig.1.

Step 4 Turn the Petri dish so the base side is down. Use a straw to cut a hole in the centre of each section of the agar in the Petri dish, as shown in Fig. 2.

Hold the straw vertically and push through the agar to the bottom of the layer. As you remove the straw twist it slightly to pull out the agar. Squeeze the end of the straw gently to push the agar you have removed onto a paper towel.

Step 5 Use a pipette to transfer three drops of solution from test-tube A into the hole in the agar in section A of the Petri dish.
Do not let the solution drip onto the surface of the agar.
Step 6 Use a clean pipette to repeat step 5 for the solution in test-tube B and the hole in the agar in section B of the Petri dish.
Step 7 Use a clean pipette to repeat step 5 for the solution in test-tube C and the hole in the agar in section C of the Petri dish. 
Step 8 Start the stop-clock and leave the Petri dish for five minutes.
Step 9 After five minutes observe the appearance of the agar around each of the holes.

Describe the appearance of the agar around the holes in A, B and C after five minutes.
...........................................................................................................................................
Question 14
Hard
Views

Views: 6,154

You are going to measure the distance moved by different concentrations of citric acid through agar.

You are provided with a Petri dish labelled agar plate.

The agar in the Petri dish contains Universal Indicator which will change colour in the presence of acid.

You should use the safety equipment provided while you are carrying out the practical work.

Step 1 Label three test-tubes A, B and C and place them in a test-tube rack.
Step 2 Make three solutions, each containing a different concentration of citric acid, in the labelled test-tubes.
Use the volumes of 5% citric acid and distilled water shown in the table to make the solutions.

Step 3 Turn the Petri dish over so the base side is up. Use a marker pen to draw three lines to divide the base into approximately equal sections. Label the sections A, B and C as shown in Fig.1.

Step 4 Turn the Petri dish so the base side is down. Use a straw to cut a hole in the centre of each section of the agar in the Petri dish, as shown in Fig.2.

Hold the straw vertically and push through the agar to the bottom of the layer. As you remove the straw twist it slightly to pull out the agar. Squeeze the end of the straw gently to push the agar you have removed onto a paper towel. 

Step 5 Use a pipette to transfer three drops of solution from test-tube A into the hole in the agar in section A of the Petri dish.
Do not let the solution drip onto the surface of the agar.
Step 6 Use a clean pipette to repeat step 5 for the solution in test-tube B and the hole in the agar in section B of the Petri dish.
Step 7 Use a clean pipette to repeat step 5 for the solution in test-tube C and the hole in the agar in section C of the Petri dish.
Step 8 Start the stop-clock and leave the Petri dish for five minutes.
Step 9 After five minutes observe the appearance of the agar around each of the holes.
Step 10 Leave the Petri dish for a further 25 minutes. During this time, continue with the other questions.
Step 11 After a total of 30 minutes use the ruler to measure the distance the citric acid has travelled from the edge of the hole in section A. You may need to use the hand lens.
Step 12 Repeat step 11 for the holes in section B and section C of the Petri dish.

Describe how you decided where to measure the distance travelled by the citric acid solution.
...........................................................................................................................................
Question 15
Medium
Views

Views: 5,823

You are going to measure the distance moved by different concentrations of citric acid through agar.

You are provided with a Petri dish labelled agar plate.

The agar in the Petri dish contains Universal Indicator which will change colour in the presence of acid.

You should use the safety equipment provided while you are carrying out the practical work.

Step 1 Label three test-tubes A, B and C and place them in a test-tube rack.
Step 2 Make three solutions, each containing a different concentration of citric acid, in the labelled test-tubes.
Use the volumes of 5% citric acid and distilled water shown in the table to make the solutions.

Step 3 Turn the Petri dish over so the base side is up. Use a marker pen to draw three lines to divide the base into approximately equal sections. Label the sections A, B and C as shown in Fig.A1.

Step 4 Turn the Petri dish so the base side is down. Use a straw to cut a hole in the centre of each section of the agar in the Petri dish, as shown in Fig.2.
Hold the straw vertically and push through the agar to the bottom of the layer. As you remove the straw twist it slightly to pull out the agar. Squeeze the end of the straw gently to push the agar you have removed onto a paper towel.
v
Step 5 Use a pipette to transfer three drops of solution from test-tube A into the hole in the agar in section A of the Petri dish. Do not let the solution drip onto the surface of the agar.
Step 6 Use a clean pipette to repeat step 5 for the solution in test-tube B and the hole in the agar in section B of the Petri dish.
Step 7 Use a clean pipette to repeat step 5 for the solution in test-tube C and the hole in the agar in section C of the Petri dish.
Step 8 Start the stop-clock and leave the Petri dish for five minutes.
Step 9 After five minutes observe the appearance of the agar around each of the holes.
Step 10 Leave the Petri dish for a further 25 minutes. During this time, continue with the other questions.
Step 11 After a total of 30 minutes use the ruler to measure the distance the citric acid has travelled from the edge of the hole in section A. You may need to use the hand lens.
Step 12 Repeat step 11 for the holes in section B and section C of the Petri dish.

Prepare a table to record your results. You should include: • the concentration of the citric acid solutions • the distance moved by each solution in the agar. Record your results in the table as you carry out the practical work
Question 16
Hard
Views

Views: 5,339

You are going to measure the distance moved by different concentrations of citric acid through agar.

You are provided with a Petri dish labelled agar plate.

The agar in the Petri dish contains Universal Indicator which will change colour in the presence of acid.

You should use the safety equipment provided while you are carrying out the practical work.
Step 1 Label three test-tubes A, B and C and place them in a test-tube rack.
Step 2 Make three solutions, each containing a different concentration of citric acid, in the labelled test-tubes.
Use the volumes of 5% citric acid and distilled water shown in the table to make the solutions.

Step 3 Turn the Petri dish over so the base side is up. Use a marker pen to draw three lines to divide the base into approximately equal sections. Label the sections A, B and C as shown in Fig.1.

Step 4 Turn the Petri dish so the base side is down. Use a straw to cut a hole in the centre of each section of the agar in the Petri dish, as shown in Fig.2.

Hold the straw vertically and push through the agar to the bottom of the layer. As you remove the straw twist it slightly to pull out the agar. Squeeze the end of the straw gently to push the agar you have removed onto a paper towel.

Step 5 Use a pipette to transfer three drops of solution from test-tube A into the hole in the agar in section A of the Petri dish.
Do not let the solution drip onto the surface of the agar.
Step 6 Use a clean pipette to repeat step 5 for the solution in test-tube B and the hole in the agar in section B of the Petri dish.
Step 7 Use a clean pipette to repeat step 5 for the solution in test-tube C and the hole in the agar in section C of the Petri dish. 
Step 8 Start the stop-clock and leave the Petri dish for five minutes.
Step 9 After five minutes observe the appearance of the agar around each of the holes.
Step 10 Leave the Petri dish for a further 25 minutes. During this time, continue with the other questions.
Step 11 After a total of 30 minutes use the ruler to measure the distance the citric acid has travelled from the edge of the hole in section A. You may need to use the hand lens.
Step 12 Repeat step 11 for the holes in section B and section C of the Petri dish.

State a conclusion for your results.
...........................................................................................................................................
Question 17
Medium
Views

Views: 6,112

You are going to measure the distance moved by different concentrations of citric acid through agar.

You are provided with a Petri dish labelled agar plate.

The agar in the Petri dish contains Universal Indicator which will change colour in the presence of acid.

You should use the safety equipment provided while you are carrying out the practical work.
Step 1 Label three test-tubes A, B and C and place them in a test-tube rack.
Step 2 Make three solutions, each containing a different concentration of citric acid, in the labelled test-tubes.
Use the volumes of 5% citric acid and distilled water shown in the table to make the solutions.

Step 3 Turn the Petri dish over so the base side is up. Use a marker pen to draw three lines to divide the base into approximately equal sections. Label the sections A, B and C as shown in Fig.1.

Step 4 Turn the Petri dish so the base side is down. Use a straw to cut a hole in the centre of each section of the agar in the Petri dish, as shown in Fig. 2.

Hold the straw vertically and push through the agar to the bottom of the layer. As you remove the straw twist it slightly to pull out the agar. Squeeze the end of the straw gently to push the agar you have removed onto a paper towel.

Step 5 Use a pipette to transfer three drops of solution from test-tube A into the hole in the agar in section A of the Petri dish.
Do not let the solution drip onto the surface of the agar.
Step 6 Use a clean pipette to repeat step 5 for the solution in test-tube B and the hole in the agar in section B of the Petri dish.
Step 7 Use a clean pipette to repeat step 5 for the solution in test-tube C and the hole in the agar in section C of the Petri dish. 
Step 8 Start the stop-clock and leave the Petri dish for five minutes.
Step 9 After five minutes observe the appearance of the agar around each of the holes.
Step 10 Leave the Petri dish for a further 25 minutes. During this time, continue with the other questions.
Step 11 After a total of 30 minutes use the ruler to measure the distance the citric acid has travelled from the edge of the hole in section A. You may need to use the hand lens.
Step 12 Repeat step 11 for the holes in section B and section C of the Petri dish.

The citric acid moves through the agar by diffusion. The diffusion coefficient is used to show the effect of concentration on diffusion. The formula to calculate the diffusion coefficient is:


Calculate the diffusion coefficient for a 10% solution of citric acid that travelled 14mm in 30 minutes.

Give your answer to two significant figures.

Space for working.


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