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Which of the following should be choosen for best yield if one
Biology Exemplar
Biology Exemplar

Class 12

NCERT Exemplar

Publisher:
NCERT
Subject:
Biology
1

Chapter 1: Reproduction in Organisms

48 questions

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2

Chapter 2: Sexual Reproduction in Flowering Plants

52 questions

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3

Chapter 3: Human Reproduction

48 questions

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4

Chapter 4: Reproductive Health

37 questions

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5

Chapter 5: Principles of Inheritance and Variation

52 questions

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6

Chapter 6: Molecular Basis of Inheritance

71 questions

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7

Chapter 7: Evolution

52 questions

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8

Chapter 8: Human Health and Disease

70 questions

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9

Chapter 9: Strategies for Enhancement in Food Production

77 questions

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10

Chapter 10: Microbes in Human Welfare

59 questions

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11

Chapter 11: Biotechnology: Principles and Processes

48 questions

Q1
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
Q2
Which of the following should be choosen for best yield if one were to produce a recombinant protein in large amounts?
Q3
Who among the following was awarded the Nobel Prize for the development of PCR technique?
View all question
12

Chapter 12: Biotechnology and its Applications

52 questions

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13

Chapter 13: Organisms and Populations

70 questions

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14

Chapter 14: Ecosystem

68 questions

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15

Chapter 15: Biodiversity and Conservation

61 questions

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16

Chapter 16: Environmental Issues

52 questions

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Question
Hard
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Solving time: 4 mins

Which of the following should be choosen for best yield if one were to produce a recombinant protein in large amounts?

A
Laboratory flask of largest capacity

B
A stirred-tank bioreactor without in-lets and out-lets

C
A continuous culture system

D
Any of the above

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Updated on: Sep 14, 2022

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Biology Exemplar (NCERT Exemplar)

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Text SolutionText solutionverified iconVerified

If any protein encoding gene is expressed in a heterologous host. it is called a recombinant protein. The cells harbouring cloned genes of interest may be grown on a small scale in the laboratory.
The cultures may be used for extracting the desired protein and then purifying it by using different separation techniques.
The cells can also be multiplied in a continuous culture system where in the used medium is drained out from one side while fresh medium is added from the other to maintain the cells in their physiologically most active log/exponential phase. This type of culturing method produces a larger biomass leading to higher yields of desired protein.
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Question Text
Which of the following should be choosen for best yield if one were to produce a recombinant protein in large amounts?
Updated OnSep 14, 2022
TopicBiotechnology: Principles and Processes
SubjectBiology
ClassClass 12
Answer TypeText solution:1 Video solution: 1
Upvotes277
Avg. Video Duration10 min