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A bacterial cell was transformed with a recombinant DNA that w
Biology Exemplar
Biology Exemplar

Class 12

NCERT Exemplar

Publisher:
NCERT
Subject:
Biology
1

Chapter 1: Reproduction in Organisms

48 questions

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2

Chapter 2: Sexual Reproduction in Flowering Plants

52 questions

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3

Chapter 3: Human Reproduction

48 questions

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4

Chapter 4: Reproductive Health

37 questions

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5

Chapter 5: Principles of Inheritance and Variation

52 questions

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6

Chapter 6: Molecular Basis of Inheritance

71 questions

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7

Chapter 7: Evolution

52 questions

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8

Chapter 8: Human Health and Disease

70 questions

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9

Chapter 9: Strategies for Enhancement in Food Production

77 questions

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10

Chapter 10: Microbes in Human Welfare

59 questions

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11

Chapter 11: Biotechnology: Principles and Processes

48 questions

Q1
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
Q2
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
Q3
Which of the following should be choosen for best yield if one were to produce a recombinant protein in large amounts?
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12

Chapter 12: Biotechnology and its Applications

52 questions

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13

Chapter 13: Organisms and Populations

70 questions

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14

Chapter 14: Ecosystem

68 questions

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15

Chapter 15: Biodiversity and Conservation

61 questions

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16

Chapter 16: Environmental Issues

52 questions

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Question
Hard
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Solving time: 4 mins

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

A
Human gene may have intron which bacteria cannot process.

B
Amino acid codons for humans and bacteria are different.

C
Human protein is formed but degraded by bacteria.

D
All of the above.

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Views: 5,353 students

Updated on: Jan 11, 2023

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Biology Exemplar (NCERT Exemplar)

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Text SolutionText solutionverified iconVerified

The process of making recombinant DNA molecule involves the introduction of a desired gene into the DNA of a host that will produce the desired protein.
Inducing a cloned eukaryotic gene to function in a prokaryotic host can be difficult sometime. The presence of long non-coding introns in eukaryotic genes may prevent correct expression of these genes in prokaryotes, which lack RNA-splicing machinery.
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Question Text
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
Updated OnJan 11, 2023
TopicBiotechnology: Principles and Processes
SubjectBiology
ClassClass 12
Answer TypeText solution:1 Video solution: 2
Upvotes390
Avg. Video Duration1 min