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What are the conditions necessary for fixation of atmospheric nitrogen by Rhizobium. What is their role in N fixation?

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Rhizobium is a symbiotic bacteria present in the root nodules of leguminous plants. The basic requirements for Rhizobium to carry out nitrogen fixation are as follows :
(a) Presence of the enzyme nitrogenase
(b) Presence of leg-haemoglobin
(c) Non-haem iron protein, ferrodoxin as the electron-carrier
(d) Constant supply of ATP
(e) ions as co-factors
Rhizobium contains the enzyme nitrogenase - a Mo-Fe protein - that helps in the conversion of atmospheric free nitrogen into ammonia.
The reaction is as follows :

The Rhizobium bacteria live as aerobes under free-living conditions, but require anaerobic conditions during nitrogen fixation. This is because the enzyme nitrogenase is highly sensitive to molecular oxygen. The nodules contain leg-haemoglobin, which protects nitrogenase from oxygen.
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Question Text
What are the conditions necessary for fixation of atmospheric nitrogen by Rhizobium. What is their role in N fixation?
TopicMineral Nutrition
SubjectBiology
ClassClass 11
Answer TypeText solution:1
Upvotes45