What are the steps required to attach a target protein?

Key Concepts: Target Protein, Attaching A Protein Explanation: Attaching a target protein involves several steps: Step by Step Solution: Step 1. Isolate the target protein from its source. Step 2. Purify the target protein to remove any impurities and ensure it is in its active form. Step 3. Prepare the surface to which the target protein will be attached. This may involve coating the surface with a linker molecule such as biotin or an antibody that can recognize and bind specifically to the target protein. Step 4. Incubate the purified target protein and the prepared surface together under appropriate conditions of temperature and pH, so that the target protein can bind to the surface. Step 5. Wash away any non-specifically bound protein to further purify the sample. Step 6. Confirm that the target protein is attached to the surface. This can be done through methods such as Western blotting or fluorescence imaging Final Answer: The steps required to attach a target protein include isolating and purifying the protein, preparing the surface, incubating the protein and the surface, washing away non-specifically bound proteins, and confirming attachment of the target protein through techniques such as Western blotting or fluorescence imaging.