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Class 12

Biology

Biotechnology

be mable for controlling the copy number of the linked ina is

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be mable for controlling the copy number of the linked ina is ne wants to recover many copies of the target DNA it shoulthe ned in a vector whose origin support high copy number gelectable marker: In addition to 'ori', the vector requities a selectable marker, which helps in identifying and eliminating nom[ransformants. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium (you will stady the process in subsequent section). Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol. tetracycline or kanamycin, etc., are considered useful selectable markers for $E$ . coli. The normal $E$ . coli cells do not carry resistantet against any of these antibiotics. $(2 re 2)$

Uploaded: 2024-01-06T11:55:18.000Z
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Updated on: Jan 6, 2024

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be mable for controlling the copy number of the linked ina is ne wants to recover many copies of the target DNA it shoulthe ned in a vector whose origin support high copy number gelectable marker: In addition to 'ori', the vector requities a selectable marker, which helps in identifying and eliminating nom[ransformants. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium (you will stady the process in subsequent section). Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol. tetracycline or kanamycin, etc., are considered useful selectable markers for

E

. coli. The normal

E

. coli cells do not carry resistantet against any of these antibiotics.

(2 re 2)

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Question Text	be mable for controlling the copy number of the linked ina is ne wants to recover many copies of the target DNA it shoulthe ned in a vector whose origin support high copy number gelectable marker: In addition to 'ori', the vector requities a selectable marker, which helps in identifying and eliminating nom[ransformants. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium (you will stady the process in subsequent section). Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol. tetracycline or kanamycin, etc., are considered useful selectable markers for $E$ . coli. The normal $E$ . coli cells do not carry resistantet against any of these antibiotics. $(2 re 2)$
Updated On	Jan 6, 2024
Topic	Biotechnology
Subject	Biology
Class	Class 12
Answer Type	Video solution: 1
Upvotes	147
Avg. Video Duration	3 min

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