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After restriction enzyme digest,the digested plasmid and PCR p
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After restriction enzyme digest,the digested plasmid and PCR product is combined, and DNA ligase is added.The mix is incubated for the ligation process to proceed.This is performed three separate times in separate tubes (reaction (Rx) 1-3).The products of each digestion and ligation reaction are run on an agarose gel and the below image is obtained. Note the PCR product is 455bp. DNA Ladder Rx.1 Rx.2 Rx.3 220C 08T 1300 a.Interpret the gel image to identify the labelled bands Band A Band B Band C 4 Band D Band E Rx1: R2: Rx3:

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After restriction enzyme digest,the digested plasmid and PCR product is combined, and DNA ligase is added.The mix is incubated for the ligation process to proceed.This is performed three separate times in separate tubes (reaction (Rx) 1-3).The products of each digestion and ligation reaction are run on an agarose gel and the below image is obtained. Note the PCR product is 455bp. DNA Ladder Rx.1 Rx.2 Rx.3 220C 08T 1300 a.Interpret the gel image to identify the labelled bands Band A Band B Band C 4 Band D Band E Rx1: R2: Rx3:
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Question Text
After restriction enzyme digest,the digested plasmid and PCR product is combined, and DNA ligase is added.The mix is incubated for the ligation process to proceed.This is performed three separate times in separate tubes (reaction (Rx) 1-3).The products of each digestion and ligation reaction are run on an agarose gel and the below image is obtained. Note the PCR product is 455bp. DNA Ladder Rx.1 Rx.2 Rx.3 220C 08T 1300 a.Interpret the gel image to identify the labelled bands Band A Band B Band C 4 Band D Band E Rx1: R2: Rx3:
TopicAll topics
SubjectBiology
ClassClass 12