A purified protein is in a Hepes (N-(2-hydroxyethyl)piperazine- $N^{'} - (2 -ethanesulfonic acid)$ ) buffer at pH 7 with $500 mm$ NaCl. A sample $(1 mL)$ of the protein solution is placed in a tube made of dialysis membrane and dialyzed against $1 L$ of the same Hepes buffer with $0 m$ . NaCl. Small molecules and ions (such as $Na^{+}, Cl^{-}$ , and Hepes) can diffuse across the dialysis membrane, but the protein cannot.(a) Once the dialysis has come to equilibrium, what is the concentration of NaCl in the protein sample? Assume no volume changes occur in the sample during the dialysis.(b) If the original 1 mL sample were dialyzed twice, successively, against $100 mL$ of the same Hepes buffer with $0 my NaCl$ what would be the final NaCl concentration in the sample?

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A purified protein is in a Hepes (N-(2-hydroxyethyl)piperazine-

N^{'} - (2 -ethanesulfonic acid)

) buffer at pH 7 with

500 mm

NaCl. A sample

(1 mL)

of the protein solution is placed in a tube made of dialysis membrane and dialyzed against

1 L

of the same Hepes buffer with

0 m

. NaCl. Small molecules and ions (such as

Na^{+}, Cl^{-}

, and Hepes) can diffuse across the dialysis membrane, but the protein cannot.(a) Once the dialysis has come to equilibrium, what is the concentration of NaCl in the protein sample? Assume no volume changes occur in the sample during the dialysis.(b) If the original 1 mL sample were dialyzed twice, successively, against

100 mL

of the same Hepes buffer with

0 my NaCl

what would be the final NaCl concentration in the sample?

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Question Text	A purified protein is in a Hepes (N-(2-hydroxyethyl)piperazine- $N^{'} - (2 -ethanesulfonic acid)$ ) buffer at pH 7 with $500 mm$ NaCl. A sample $(1 mL)$ of the protein solution is placed in a tube made of dialysis membrane and dialyzed against $1 L$ of the same Hepes buffer with $0 m$ . NaCl. Small molecules and ions (such as $Na^{+}, Cl^{-}$ , and Hepes) can diffuse across the dialysis membrane, but the protein cannot.(a) Once the dialysis has come to equilibrium, what is the concentration of NaCl in the protein sample? Assume no volume changes occur in the sample during the dialysis.(b) If the original 1 mL sample were dialyzed twice, successively, against $100 mL$ of the same Hepes buffer with $0 my NaCl$ what would be the final NaCl concentration in the sample?
Topic	All topics
Subject	Biology
Class	Class 11

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