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25. Choose the correct pair from the following.
(a) Ligases
- Join the

25. Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

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in of replication (ori): This is a sequence from where in starts and any piece of DNA when linked to this sequence be made to replicate within the host cells. This sequence is also onsible for controlling the copy number of the linked DNA. So, ewants to recover many copies of the target DNA it should be oned in a vector whose origin support high copy number.
  1. dectable marker, which : In addition to 'orl', the vector requires a ansformants and seletich intifying and eliminating nonansformants. Transformation permitting the growth of the cece of DNA is introduced in a host bacterium (you will stuch a process in subsequent section). Normally, the genes enco resistance to antibiotics such as ampicillin, chloramphe tetracycline or kanamycin, etc., are considered useful selectabl. markers for . coli. The normal . colicells do not carry resistance Identroging Now against any of these antibiotics.
  2. Cloning sites: In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning (Figure 9.4). The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector . The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from non-recombinant ones by PBR 322 prasmid plating the transformants on
  3. Figure 9.4 E. coli cloning vector showing restriction sit (Hind III, EcoR I, BamH I, Sal Puu II, Pst I, Cla I), ori a antibiotic resistance gen and tet ). rop codes the proteins involved in replication of the plasmid.
  4. tetracycline containing medium. The transformants growing on ampicillin containing medium are then transferred on a medium containing tetracycline. The recombinants will grow in ampicillin containing medium but not on that containing tetracycline. But, non-recombinants will grow on the medium containing both the antibiotics. In this case, one antibiotic resistance gene helps in selecting the transformants, whereas the other antibiotic resistance
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Question Text
25. Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA
Updated OnApr 25, 2024
TopicBiotechnology
SubjectBiology
ClassClass 12
Answer Type Video solution: 1
Upvotes148
Avg. Video Duration1 min