1. You have cloned the NovP1 gene and raised a monoclonal antibody against the NovP1 protein. Briefly explain how you will use this antibody to: a) purify NovP1 and its interacting proteins (start with HSC cell lysate). b) show that you purified NovP1 (hint: it uses a gel). a) To purify NovP1 and its interacting proteins from the HSC cell lysate, you will use the monoclonal antibody against NovP1 in an immunoprecipitation assay. First, you will incubate the HSC cell lysate with the monoclonal antibody, allowing the antibody to bind specifically to NovP1 and any interacting proteins. Then, you will use protein A or protein G beads to capture the antibody-protein complexes. By washing the beads, you can remove any non-specifically bound proteins. Finally, you will elute the purified NovP1 and its interacting proteins from the beads, obtaining a purified sample for further analysis. b) To show that you have purified NovP1, you can use a gel electrophoresis technique. First, you will load the purified NovP1 sample onto a polyacrylamide gel. Then, you will apply an electric current to the gel, causing the proteins to migrate through the gel based on their size. Since NovP1 has been purified, it should appear as a distinct band on the gel. To visualize the protein, you can stain the gel with a protein-specific dye, such as Coomassie Brilliant Blue. The stained gel can then be analyzed using imaging techniques, such as densitometry, to quantify the amount of purified NovP1 present.