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Which of the following is the mismatched pair?

A
Mineral element- nitrogen
Form that is absorbed by plant-

B
Mineral element- phosphorus
Form that is absorbed by plant-

C
Mineral element- sulphur
Form that is absorbed by plant-

D
Mineral element- iron
Form that is absorbed by plant-

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Plants obtain sulphur in the form of sulphate ( ).
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(a) From this plot, estimate Km and Vmax for carbonic anhydrase. Make sure to include appropriate units. (b) Using the values that you estimated in part (a), calculate the initial velocity (V0) of the reaction when [CO2] is 5 mM. (c) Calculate the concentration of [CO2] at which V0 will be 95% of Vmax. (d) In the space below, convert the data from the researchers' plot into a double-reciprocal plot and indicate the slope and x-axis and y-axis intercepts. (e) The researchers work to develop a mutant of this enzyme with a specificity constant that approaches "catalytic perfection", such that it is only limited by the rate of enzyme substrate diffusion (in this case 108 M-1 s-1). If the Km of the mutant enzyme remains the same as the wildtype (shown above), and the molecular weight of the mutant enzyme is 30 kDa, how many grams of CO2 would 5 g of enzyme need to turn over in 1 minute to reach catalytic perfection? (f) Considering that 10 g of the wild type enzyme was used to generate the data in the plot for part (a), calculate cat and the specificity constant for the wild type enzyme. The molecular weight of the wild type enzyme is 30 kDa and the volume of the reaction solution for the experiment in part (a) is 1 L. Assuming that the researchers from part (e) are successful in creating a mutant enzyme that achieves "catalytic perfection" with a specificity constant of 108 M-1 s-1, compare the cat and the specificity constant for the wild type and mutant enzymes. How many more molecules of substrate would be converted to product in a minute by a single mutant enzyme as compared to a single wild type enzyme? Please report both the actual number of molecules and the factor increase. 2. A group of researchers has been working with the enzyme carbonic anhydrase which catalyzes the following reaction to hydrate cO2 and allow for its transport from the tissues to the lungs: H2O + CO2= H++HCO3 The researchers measure the dependence of the initial velocity of the enzyme catalyzed reaction on substrate concentration and a plot of their data is shown below: 8.0 6.0 Vo 4.0 - (mM *min-1) 2.0 of 15 30 45 60 75 [COz] (mM)
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Question Text
Which of the following is the mismatched pair?
Updated OnOct 24, 2022
TopicMineral Nutrition
SubjectBiology
ClassClass 11
Answer TypeText solution:1 Video solution: 1
Upvotes205
Avg. Video Duration20 min