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Significance of heat shock method in bacterial transformation
Biology Exemplar
Biology Exemplar

Class 12

NCERT Exemplar

Publisher:
NCERT
Subject:
Biology
1

Chapter 1: Reproduction in Organisms

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2

Chapter 2: Sexual Reproduction in Flowering Plants

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3

Chapter 3: Human Reproduction

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4

Chapter 4: Reproductive Health

37 questions

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5

Chapter 5: Principles of Inheritance and Variation

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6

Chapter 6: Molecular Basis of Inheritance

71 questions

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7

Chapter 7: Evolution

52 questions

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8

Chapter 8: Human Health and Disease

70 questions

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9

Chapter 9: Strategies for Enhancement in Food Production

77 questions

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10

Chapter 10: Microbes in Human Welfare

59 questions

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11

Chapter 11: Biotechnology: Principles and Processes

48 questions

Q1
An antibiotic resistance gene in a vector usually helps in the selection of
Q2
Significance of heat shock method in bacterial transformation is to facilitate.
Q3
Significance of heat shock method in bacterial transformation is to facilitate.
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12

Chapter 12: Biotechnology and its Applications

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13

Chapter 13: Organisms and Populations

70 questions

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14

Chapter 14: Ecosystem

68 questions

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15

Chapter 15: Biodiversity and Conservation

61 questions

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16

Chapter 16: Environmental Issues

52 questions

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Question
Easy
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Solving time: 2 mins

Significance of heat shock method in bacterial transformation is to facilitate.

A
Binding of DNA to the cell wall

B
Uptake of DNA through membrane transport proteins

C
Uptake of DNA through transient pores in the bacterial cell membrane

D
Expression of antibiotic resistance gene

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Updated on: Nov 27, 2022

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Text SolutionText solutionverified iconVerified

In chemical method, the cell is treated with specific concentration of a divalent cation such as calcium to increase pore size in cell wall. The cells are incubated with recombinant DNA on ice, followed by placing them briefly at 42°C and then putting it back on ice. This is called heat shock method. The bacteria now takes up the recombinant DNA.
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Question Text
Significance of heat shock method in bacterial transformation is to facilitate.
Updated OnNov 27, 2022
TopicBiotechnology: Principles and Processes
SubjectBiology
ClassClass 12
Answer TypeText solution:1 Video solution: 1
Upvotes144
Avg. Video Duration20 min