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An enzyme catalysing the removal of nucleotides from the ends of DNA is
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(b) Restriction enzymes belongs to a class of enzymes called nucleases and are of two types
(i) Exonucleases remove nucleotides from the ends of the DNA.
(ii) Endonucleases make cuts at specific positions within the DNA.
DNA ligase is a sealing enzymes (also called as genetic gum), which is responsible for joining of two individual fragments of DNA, whereas Hind II is first discovered restriction endonuclease enzyme.
(i) Exonucleases remove nucleotides from the ends of the DNA.
(ii) Endonucleases make cuts at specific positions within the DNA.
DNA ligase is a sealing enzymes (also called as genetic gum), which is responsible for joining of two individual fragments of DNA, whereas Hind II is first discovered restriction endonuclease enzyme.
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Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type 11 and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.
Which type of restriction endonucleases is used most in genetic engineering?
(A) Type I
(B) Type II
(C) Type III
(D) Type IV
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Question Text | An enzyme catalysing the removal of nucleotides from the ends of DNA is |
Topic | Biotechnology: Principles and Processes |
Subject | Biology |
Class | Class 12 |
Answer Type | Text solution:1 |
Upvotes | 115 |