Why were the same restriction enzymes (BamHI and HindIII) added to a solution containing both the pKAN-R and pARA plasmids?

1. Because we used two different plasmids, and each plasmid requires a different restriction enzyme.
2. To create the same sticky ends on fragments from both plasmids that can later be joined together.
3. Adding both enzymes would protect the plasmid from other viruses or contaminants present in the tubes and solutions.
4. To create fragments of the same size.

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