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the source DNA with the sam molecule cannot be created. restriction enzyme, the recombinant vecy the following restriction endonucleases results in the fragments of $DNA$. These fragmeng into a vector. can be separated by a technique known as gel electrophoresis. Singen (i) Origin of replication s by forcing them to move towards the anode under an electric field throug can be mad a medium/matrix. Nowadays the most commonly used matrix is ag responsible if one want: which is a natural polymer extracted from sea weeds. (ii) Selectable by the agarose gel. Hence, the smaller the fragment size, the farther it moves. Look at the Figure 11.3 and guess at which end of the gel the sample was loaded. The separated DNA fragments canbe visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV

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Updated on: Mar 18, 2023