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Texts: Exercise 1 Activity 3 (Simulating Osmotic Pressure) in PhysioEx.

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  2. For Run 4 (MWCO 100 amu, 1 mM of glucose on the left, water on the right), the osmotic pressure that developed was mmHg.
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  4. For Run 7 (MWCO 200 amu, 9 mM albumin on the left, 10 mM of glucose on the right), the measurement for the osmotic pressure was mmHg.
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Texts: 1. Genes for the synthesis of branched chain amino acids (BCAAs) are encoded by the ilv-leu operon (ilv-leu is a mashup of isoleucine, valine, and leucine). Its regulation is quite complicated, but it involves the action of two separate proteins: 1. A protein named codY binds to the operator regions, preventing transcription if BCAAs are abundantly available in the environment. 2. The operon is under negative control by a protein named TnrA, which responds to nitrogen availability in the environment in such a way that only high levels of nitrogen turn on the expression of the operon. A. Describe the action of codY (how does it interact with the RNA polymerase and how does it react to its environmental trigger)? Is codY involved in positive or negative control? Is the operon repressed or activated by BCAAs in the environment? Explain your answers by comparing this control mechanism to the control of the trp operon. B. Describe the action of TnrA (how does it interact with the RNA polymerase and how does it react to its environmental trigger)? Is the TnrA protein a transcriptional repressor or activator? Is the operon repressed or activated by nitrogen availability in the environment? Explain your answers by comparing this control mechanism to the control of the trp operon. 2. In addition to codY and TnrA, the ilv-leu operon is controlled by a third protein called Catabolite control protein A (CcpA). Its mechanism of action (how CcpA interacts with the RNA polymerase enzyme) is the same as the Catabolite activator protein (Cap) in the lac operon. A. What is the function of Cap in the regulation of the lac operon? Is this an example of positive or negative control? What is the environmental trigger to which it responds, and what does Cap do upon binding this trigger? B. The lac operon simultaneously gauges the availability of lactose and glucose in the environment. Which of these functions would be lost if Cap were mutated or missing?
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Question Text
Texts: Exercise 1 Activity 3 (Simulating Osmotic Pressure) in PhysioEx.
TopicAll topics
SubjectBiology
ClassClass 12