Texts: Design forward and reverse primers to the following DNA fragments. Primers are typically 20 nucleotides in length with annealing temperatures around 50-60°C, but only design these primers to be six nucleotides in length for simplicity, and write all primers 5'-3'. Also, calculate each primer's Tm and the annealing temperature that should be used in PCR. 5'-GTACTAGTAGACCGTATGCCGCAGTA-3' Forward: 5'-GTACTA-3' Tm: 16°C Reverse: 5'-TACTGC-3' Tm: 18°C Annealing Temp: 13°C 5'-GCTGTAAGAGGATTTACTACCGTCCA-3' Forward: 5'-GCTGTA-3' Tm: 18°C Reverse: 5'-TGGACG-3' Tm: 20°C Annealing Temp: 15°C

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