QUESTION 2 [20 MARKS] (a) Plant pathogen detection is important as the first step in managing a plant disease in greenhouses, field conditions, and at the country borders. However, previous instruments, being not suitable for in-situ analysis. Discuss the process develop a biosensor to detect pathogens in plants from Figure 2 [10 Marks] [CO2, PO3, C3] Figure 2 (b) Plants with crown gall have swollen knots, called galls, near the crown and sometimes on the roots and twigs (Figure 3). As the disease progresses, the galls can totally encircle the trunks and branches, cutting off the flow of sap that nourishes the plant. The findings from Crown Gall disease have led to the discovery of gene transfer into plant cells. Using a specific microorganism, outline a specific technique to transform diagram. [10 Marks]

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