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Solving time: 1 mins

Which of the following statement is correct?

A
All bacteria are heterotrophic

B
Bacteria are either heterotrophic or chemoautotrophic

C
Bacteria can also be photoautotrophic

D
Bacteria are either photoautotrophic or chemoautotrophic

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Correct answer is C
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Problem: Cloning Challenge You are doing an internship at a biotech company that develops weight loss drugs. They have identified a hormone called SKNY1 that decreases feelings of hunger. Early evidence in animal trials indicates that the hormone may be an effective treatment for weight loss. The company would like to produce this hormone for a clinical trial to see if it is effective for weight loss in clinically obese individuals. This worksheet will guide you through the steps that you can use to create a vector that can be used for production of the hormone in bacteria. Step 1: Design primers to amplify the SKNY1 gene. 1. Below is the region of DNA containing the SKNY1 gene. Underline the sequences that could be used as primers. You will need a forward primer and a reverse primer. 5'-CCAATGCCGAGCTGTGCCTAGGCTAGCGTGTCGCTTGTA.........(600 bps).........GGGCTGCTATCGATCGATGCTGCACGCTAGTCTAA-3' 3'-GGTTACGGCTCGACACGGATCCGATCGCACAGCGAACAT.........(600 bps).........CCCGACGATAGCTAGCTACGACGTGCGATCAGCTT-5' Forward primer: 5' - Reverse primer: 3' - Step 2: PCR-amplify the target sequence. 2. Next, you will need to PCR-amplify the target sequence to clone it into the plasmid vector. Should you use cDNA library or a genomic DNA library as your insert source? Explain your answer. Step 3: Clone the insert DNA (SKNY1 sequence) into the cloning vector. 3. Next, you need to clone the insert into a plasmid vector. Should you use a cloning vector or an expression vector? Explain your answer. 4. Below is the map for the plasmid vector that you can use for cloning of the SKNY1 gene. This vector has the regulatory region from the lac operon (promoter and operator region) followed by a multiple cloning site (MCS). How will you use select specific restriction enzymes to ensure that the insert DNA (SKNY1 sequence) and the plasmid will ligate together in the correct orientation? Step 4: Transformation of E. coli with the vector + insert 5. Why would you need to transform the plasmid into bacteria? Step 5: Expression of the SKNY gene 6. IPTG (Isopropyl β-D-1-thiogalactopyranoside) is a compound that mimics lactose. Predict what will happen if you grow the bacteria (containing the above plasmid) in a medium containing ampicillin and IPTG.
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Question Text
Which of the following statement is correct?
Updated OnFeb 24, 2024
TopicBiological Classification
SubjectBiology
ClassClass 11
Answer TypeText solution:1 Video solution: 13
Upvotes1323
Avg. Video Duration8 min