The figure below shows three steps (A, B, C) of polymerase chain reaction (PCR). Select the option giving correct identification together with what it represents?

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Filo · Accepted Answer

PCR or polymerase chain reaction is the technique employed for the amplification of the gene of interest. The image shows 3 steps of the PCR cycle, namely denaturation, annealing, and extension. Part A represents the step of denaturation, where the dsDNA is divided into two ssDNA under high temperature. For denaturation to occur, an approximate temperature of 90-92oC is required.Whereas, part B represents the attachment of short oligonucleotide sequences called primer by a process called annealing and part C represents the extension of the DNA segment by the addition of nucleotides by the action of the thermostable polymerase called Taq polymerase. Thus, the correct answer is option A.

Question Text	The figure below shows three steps (A, B, C) of polymerase chain reaction (PCR). Select the option giving correct identification together with what it represents?
Answer Type	Text solution:1
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The figure below shows three steps (A, B, C) of polymerase chain reaction (PCR). Select the option giving correct identification together with what it represents?

Text solutionVerified

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