World's only instant tutoring platform
dropdown-logo
Get 2 FREE Instant-Explanations on Filo with code FILOAPP
Question

Expand ELISA. On what principle is ELISA test based? List two ways by which an infection can be detected by this test.

tutor 0tutor 1tutor 2
Found 7 tutors discussing this question
Discuss this question LIVE
12 mins ago

Text SolutionText solutionverified iconVerified

ELISA or Enzyme-Linked Immunosorbent Assay is an immunoassay technique used for the detection of diseases. ELISA is based on the principle that specific antibodies bind to their target antigen i.e it is based on the principle of antigen-antibody interaction. Only when the interaction occurs the substrate will be able to bind to the enzyme and substrate conversion will be seen, thus giving a positive result.
Two ways by which the infection can be detected by this method are -
i) Direct ELISA: in which the sample is first added and then the enzyme-conjugated antibody is added. If an interaction occurs reaction will be seen on substrate addition.
ii) sandwich ELISA- in which the sample is sandwiched between two layers of antibodies and if interaction occurs substrate reaction will be seen on addition of a conjugated antibody and substrate.HIV and rotavirus infection can be detected by this technique.
Was this solution helpful?
153
Share
Report
One destination for complete JEE/NEET preparation
One destination to cover all your homework and assignment needs
Learn Practice Revision Succeed
Instant 1:1 help, 24x7
Instant 1:1 help, 24x7
60, 000+ Expert tutors
60, 000+ Expert tutors
Textbook solutions
Textbook solutions
Big idea maths, McGraw-Hill Education etc
Big idea maths, McGraw-Hill Education etc
Essay review
Essay review
Get expert feedback on your essay
Get expert feedback on your essay
Schedule classes
Schedule classes
High dosage tutoring from Dedicated 3 experts
High dosage tutoring from Dedicated 3 experts
Trusted by 4 million+ students
filo Logo
Doubt Icon Doubt Icon

Stuck on the question or explanation?

Connect with our biology tutors online and get step by step solution of this question.

231 students are taking LIVE classes
Question Text
Expand ELISA. On what principle is ELISA test based? List two ways by which an infection can be detected by this test.
Answer TypeText solution:1
Upvotes153