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Biotechnology: Principles and Processes

Processes of Recombinant DNA Technology

Assertion: Taq Polymerase is involved in PCR technique Reason:

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Assertion: Taq Polymerase is involved in PCR technique
Reason: This enzyme remain active during the high temperature including denaturation of double stranded DNA.

Both Assertion and Reason are correct and Reason is the correct explanation for Assertion

Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion

Assertion is correct but Reason is incorrect

Both Assertion and Reason are incorrect

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Assertion: Taq Polymerase is involved in PCR technique
Reason: This enzyme remain active during the high temperature including denaturation of double stranded DNA.

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Text solutionVerified

Taq Polymerase is used in PCR (Polymerase Chain Reaction) to extend the primers that anneal to the ends of template DNA and complete the replication of the rest DNA strands. Hence, assertion is correct.
Taq Polymerase is derived from a thermophilic bacterium Thermus aquaticus. This bacterium grows naturally in hot springs at a temperature of 90° C and hence, the enzyme Taq Polymerase doesn't get denatured by high temperature in the PCR steps. Hence, reason is also correct and is correct explanation for assertion.

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Question Text	Assertion: Taq Polymerase is involved in PCR technique Reason: This enzyme remain active during the high temperature including denaturation of double stranded DNA.
Topic	Biotechnology: Principles and Processes
Subject	Biology
Class	Class 12
Answer Type	Text solution:1
Upvotes	115

Assertion: Taq Polymerase is involved in PCR technique
Reason: This enzyme remain active during the high temperature including denaturation of double stranded DNA.

Text solutionVerified

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Assertion: Taq Polymerase is involved in PCR technique
Reason: This enzyme remain active during the high temperature including denaturation of double stranded DNA.